I add to the list as I think of or find protocols, media recipes, and the like. Check back from time to time. If you want a protocol that I have used in a paper, but that I do not have here, please contact me.
Standard competition protocol and flow used to assay relative fitness differences between clones and populations
| competition_flow.doc |
Pictorial protocol for gene replacement using the pKOV method
| flow_for_allele_replacement_with_pkov.docx |
Protocol for making precise deletions using pKOV - From Ben Kerr's lab via Jeff Morris
| making_precise_deletions_using_pkov_-_from_ben_kerrs_lab_via_jeff_morris.docx |
Protocol and Description for Fluctuation Tests Used in Experiments into Contingency's Role in Cit+ Evolution
| cit_fluctuation_test_series_description_and_flow_used_in_contingency_experiments.doc |
Protocol for Gene Gorging (Warning: I have not found Gene Gorging to be terribly efficient.)
| gene_gorging.doc |
Media Recipes
"Luria-Bertani" (LB) Medium:
Per liter:
10 g tryptone
5 g yeast extract
5 g NaCl
1 L dH20
If making plates:
16 g agar
1 mL antifoam
"Davis-Mingioli" (DM) Minimal Medium
Per liter:
7g Potassium phosphate (dibasic trihydrate)
2 g Potassium phosphate (monobasic anhydrous)
1 g Ammonium Sulfate
0.5 g Sodium Citrate
1 L dH2O
After autoclaving add:
1 mL 10% Magnesium Sulfate
1 mL 0.2% Thiamine (Vitamine B1)
For DM25 glucose, the standard medium used for the LTEE, add 0.25 mL 10% glucose. Any other sugar may be added at any other concentration one might wish.
Per liter:
10 g tryptone
5 g yeast extract
5 g NaCl
1 L dH20
If making plates:
16 g agar
1 mL antifoam
"Davis-Mingioli" (DM) Minimal Medium
Per liter:
7g Potassium phosphate (dibasic trihydrate)
2 g Potassium phosphate (monobasic anhydrous)
1 g Ammonium Sulfate
0.5 g Sodium Citrate
1 L dH2O
After autoclaving add:
1 mL 10% Magnesium Sulfate
1 mL 0.2% Thiamine (Vitamine B1)
For DM25 glucose, the standard medium used for the LTEE, add 0.25 mL 10% glucose. Any other sugar may be added at any other concentration one might wish.